Coding

Part:BBa_K2220008:Design

Designed by: Nan Rong   Group: iGEM17_BNU-China   (2017-10-19)


Display CBH protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 325
    Illegal NheI site found at 1239
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 388
    Illegal BamHI site found at 1113
    Illegal XhoI site found at 1459
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1528
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1510


Design Notes

We have done the codon optimization.


Source

https://www.ncbi.nlm.nih.gov/protein/453223?report=genbank&log$=protalign&blast_rank=1&RID=X3B0MN08015

References